Coding

Part:BBa_K1686047:Design

Designed by: Jean Descarpentrie   Group: iGEM15_Bordeaux   (2015-09-12)

crdC gene with codon optimisation for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 361
    Illegal AgeI site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene of CrdC comes from Agrobacterium sp. ATCC31749 and we would like to use it in E.coli, so we fixed the bias codon by using the software of Integrated DNA Technologies, Inc for codon optimization.


Source

The nucleotide sequence of CrdC comes from GenBank under the accession number AECL01000028.1


References

Stasinopoulos SJ, et al. (1999). "Detection of two loci involved in (1→3)-β-glucan (curdlan) biosynthesis by Agrobacterium sp. ATCC31749, and comparative sequence analysis of the putative curdlan synthase gene." Glycobiology 9:31–41.